Fertilization is necessary for sexual reproduction of plants. Many agents like wind water birds bring male and female sex cells together for fertilization to occur. However every cell has full genetic stock. Only the necessary material is used in the differentiation to form specific organs and the rest goes in dormant stage. It means that given proper conditions it should be possible to rear a full plant from any cell. This is the principal of tissue culture.

Many cells have two pairs of genes one from the Father and the other from mother. Maheshwari discovered it is possible to do tissue culture from cells with a single pair. He performed some careful experiments and demonstrated how this is achieved. In fact when one doubles a single pair of genes one gets two sets that look precisely alike. Maheshwari said that it is therefore possible to use tissue culture to generate plants of desired (tailormade )charachteristics. Moreover such plants will have pure stock of genetic information since there would be no mixing.

Tissue culture has contributed much to the knowledge of the factors from free cells of the plant. The storage route of the cultivated carrot produce a a broad zone.

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